351: Resistance Reboot

TWiM discusses the use of bacteriophage-loaded microneedle patches for targeted and minimally disruptive foodborne pathogen decontamination, and a conjugal gene drive-like system that efficiently suppresses antibiotic resistance in bacteria. Become a patron of TWiM.   Links for this episode:   Bacteriophage-loaded microneedle patches for food (Sci Adv) Gene drive to suppress antibiotic resistance (npj antimicrob and resistance) CRISPR gene drives (Syntego) Bier laboratory Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments to [email protected]

Nancy and Maggie join TWiM to share how and why they created a freely available ebook of TWiM-based science literacy resources and classroom exercises that support teaching across key microbiology and molecular biology topics. Become a patron of TWiM. Links for this episode: Podcast annotation and resources in microbiology (Iowa State U) Curriculum guidelines for undergraduate microbiology (ASM) Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments to [email protected]

TWiM explains how mechano-bactericidal surfaces made from diverse materials and patterned with spikes kill bacteria on contact, and virus-host evolution is reshaped by microgravity aboard the International Space Station. Become a patron of TWiM. Links for this episode: Mechano-Bactericidal Surfaces (Adv Sci) Piercing pathogens (ASM) Natural bactericidal surfaces (Small) Virus-host evolution in microgravity (PLoS Biol) Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments to [email protected]

TWiM explains the finding that owning a dog during adolescence alters the microbiota and improves mental health, and the molecular basis for multidrug efflux by an anaerobic-associated resistance-nodulation-cell division transporter. Hosts: Vincent Racaniello, Michael Schmidt, Michele Swanson and Petra Levin. Become a patron of TWiM. Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Links for this episode Dog ownership and the microbiome during adolescence (iScience) Molecular basis for multidrug efflux (Nat Comm) Take the TWiM Listener survey! Send your microbiology questions and comments (email or recorded audio) to [email protected]

TWiM explains how S. aureus pathogenicity is a dynamic, niche-specific choreography that constantly recalibrates in response to the host microenvironment, and short chain fatty acids produced by commensal microbiota reduces its competitive fitness.   Hosts: Michael Schmidt, Michele Swanson and Petra Levin. Guest: Mark O. Martin   Become a patron of TWiM. Links for this episode: Niche-specific fitness of S. aureus at the wound edge (Nat Comm) Commensal derived short chain fatty acids attenuate S. aureus (mBio) Ditch the term pathogen (Nature)   Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments to [email protected]

TWiM explains how competition for nutrients anticipates and potentially mitigate drug side effects on the gut microbiota, and metabolic control of porin permeability influences antibiotic resistance. Hosts:  Vincent Racaniello, Michael Schmidt and Petra Levin Guest: Mark O. Martin Become a patron of TWiM.   Links for this episode: Nutrient competition predicts drug effects on microbiota (Cell) Metabolic control of porin permeability influences antibiotic resistance in E coli (Nat Micro) Santiago Cano-Muniz   Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments to [email protected]  

TWiM explains a biological mechanism that links sulfur and iron cycling in anoxic environments, and "swashing," a form of surface movement in which bacteria migrate without active propulsion. Hosts:  Michael Schmidt, Michele Swanson, and Petra Levin Guest: Mark O. Martin Subscribe to TWiM (free) on Apple Podcasts, RSS, or by email. Links for this episode Sulfur and iron cycling in anoxic environments (Nature) Bacterial surface migration by swashing (J Bacteriol) Become a Patron of TWiM! Music used on TWiM is by Ronald Jenkees. Send your microbiology questions and comments to [email protected]

TWiM reveals the bacteria, acids, and enzymes behind yogurt made with ants, and a defined set of microbes that reproduces attributes of fine flavor chocolate fermentation   Hosts:  Vincent Racaniello, Michael Schmidt, and Petra Levin Guest: Mark O. Martin Become a patron of TWiM. Links for this episode: Making yogurt with ants (iScience) The Alchemist Restaurant How to make classic tuiles (MasterClass) Defined microbes for chocolate (Nat Micro) Microbial chocolatiers of fine flavour (Nat Micro) Blind taste every chocolate bar (YouTube)   Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments to [email protected]

TWiM explores two different ways that bacteria defend against phages through the synthesis of cyclic nucleotides. Hosts:  Michael Schmidt and Mark O. Martin   Become a patron of TWiM. Links for this episode: Decoy cyclic nucleotides in phage defense (Nature) CRISPR defense with nucleotides (Nature) A decoy defence molecule to set a trap for viruses (Nature) Cyclic oligonucleotide-based antiphage signaling system immunity (Curr Opin Immunol)   Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments to [email protected]

TWiM explains studies that show that the oral bacterial and fungal microbiome are risk factors for pancreatic cancer, and the gut microbiome and pyruvate metabolism of older adults are a link between sleep quality and frailty. Hosts: Vincent Racaniello, Michael Schmidt, and Michele Swanson. Subscribe to TWiM (free) on Apple Podcasts, Spotify, Android, RSS, or by email. Become a patron of TWiM. Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Links for this episode Oral microbiome and pancreatic cancer risk (JAMA Oncol) 2025 Cancer Facts & Figures (ACS) Periodontal Disease: The Good, The Bad, and The Unknown (Front Cell Infect Micro) Socransky's disease complexes (Microorg) Microbiome, pyruvate metabolism, sleep quality, and frailty (Cell Rep) Pittsburgh Sleep Quality Index (pdf) How to score the sleep quality index (pdf) Take the TWiM Listener survey! Send your microbiology questions and comments (email or recorded audio) to [email protected]