Episode 39: Spatial proteomics at Pixelgen

Join us to hear Phil Ewels chat with Maxime Garcia and Franziska Bonath about how nf-core started at the National Genomics Infrastructure (NGI) at SciLifeLab in Sweden.We chat about how the nf-core/sarek pipeline grew alongside and eventually joined nf-core,as well as the early days of nf-core bytesize talks and outreach organisation. Links: - SciLifeLab - National Genomics Infrastructure - Maxime's Nextflow Summit Boston 2023 presentation about Sarek - nf-core bytesize talks

Tune in to hear all about Pixelgen Technologies - creators of a novel technology for doing single-cell spatial proteomics. We chat about how this fascinating technology works, what you can do with the data and why they chose to develop their analysis suite as an open-source nf-core pipeline. Links: Software: nf-core/pixelator pipeline Pixelator Python package Science: Nature Methods publication: “Molecular pixelation: spatial proteomics of single cells by sequencing” Presentations: Nextflow Summit 2022 talk: “Pixelgen Technologies ❤︎ Nextflow” Nextflow Summit 2024 Boston talk: “Nextflow allows Pixelgen innovation engine to reach the market quicker” Nextflow Summit 2024 Barcelona Pixelgen: Pixelgen Technologies homepage @PixelgenTech on twitter / X and LinkedIn BioLizard: BioLizard homepage @BioLizard_nv on twitter / X and LinkedIn Chapters: Start (00:00) Welcome (00:09) Introductions (00:50) Intro to Pixelgen Technologies (01:41) Lab methods (03:08) Nature Methods publication (06:15) Data visualisation (09:35) Working with single cell data (12:29) Cell interactions (15:06) Why Pixelgen chose open source (16:05) Pixelator Python package (20:10) Building within nf-core (21:40) Florian - Biolizard (23:01) Pipeline overview (25:19) Using nf-core from a commercial setting (26:50) Coming out of stealth (28:53) Extending Pixelator (32:24) Dealing with customers (34:58) Looking to the future (39:38) Interactive analysis (41:32) Advice for others (43:12) Pixelgen at the Nextflow Summit (45:16)

Evan Floden (CEO and co-founder of Seqera) joins Phil Ewels (Product Manager for OSS at Seqera) to talk about two of the big announcements at the recent Nextflow Summit Boston 2024 - Seqera Pipelines and Seqera Containers. We dive into what these two new community offerings are, why we built them and how they work. Links: https://seqera.io/pipelines/ https://seqera.io/containers/ Announcement blog post Nextflow Summit Boston 2024 agenda Summit YouTube playlist Nextflow 24.04 blog post Timeline: 00:00 - Introduction 00:37 - Nextflow Summit Boston 2024 01:14 - Summit talks available online 01:57 - Summit announcements 04:32 - Seqera Pipelines - motivations 05:59 - Seqera Pipelines - first look 06:48 - Testing requirements for community pipelines 10:05 - How we tested pipelines 11:53 - SRA-Explorer tangent 12:34 - Seqera Pipelines - live demo 16:14 - Getting Nextflow launch commands 19:12 - Seqera Containers - intro 24:03 - Seqera Containers - live demo 27:13 - Containers - ARM cpu arch builds 28:33 - Singularity containers 29:09 - Wave CLI 31:19 - Nextflow with Seqera Containers 33:22 - Seqera Containers - future usage 35:09 - Where to read more 36:53 - Thank you to the community

Join us as we discuss the brand new @nf-core / EuroFaang pre-print! 🔥 We chat to some of the key authors, covering the background to the paper, the main messages and how EuroFAANG and nf-core have collaborated. 🧬 👩🏻‍🔬

Join us in this episode of the Channels podcast for a closer look at the Nextflow Ambassadors Program. Marcel Ribeiro-Dantas and Geraldine Van der Auwera discuss the origins of the program, recent highlights and next steps, including the open call for new Ambassadors, which will close June 14. Relevant links: Call for applications: https://www.nextflow.io/ambassadors.html Original announcement: https://www.nextflow.io/blog/2023/introducing-nextflow-ambassador-program.html Marcel’s favorite Ambassador-contributed Nextflow blog post: https://www.nextflow.io/blog/2024/reflections-on-nextflow-mentorship.html

This podcast episode is quite different to normal. Rather than an interview or a discussion, you'll get to watch as Ben Sherman guides Phil Ewels through the process of creating a new Nextflow Plugin from scratch. Right from the first line of code to creating a release and publishing the plugin. Nextflow plugins have been getting more attention lately and we've been getting a lot of questions in the community. There is better documentation and resources planned, but in the mean time we hope that this can be a useful guide for anyone curious in how they work, and interested in getting started. After this we will be back to our usual routine in the podcast, but we'd love to hear what you thought of this taster! 00:00:00 Welcome 00:03:27 Nextflow docs 00:08:34 Starting by forking nf-hello 00:10:32 Overview of project files 00:17:13 Trying a first compile 00:18:12 Different publishing methods 00:19:54 nf-boost local publish method 00:23:05 Trying the new compile 00:24:00 Running locally with the plugin 00:27:32 Looking at the nf-hello plugin code 00:39:29 Deleting files we don't need 00:42:34 Finding event names in the Nextflow source 00:47:46 Writing some custom functions 00:49:41 Testing our new code 00:51:51 Modifying the test pipeline to create files 00:54:14 Coding up the JSON output 00:59:20 Looking at nf-prov code for BCO files 01:05:30 Testing JSON output 01:09:39 Automatic work dir cleanup / nf-boost 01:12:17 Publishing a release on GitHub 01:19:07 Custom plugin repositories 01:26:47 Publishing for all Nextflow users 01:29:52 Conclusion and end

In this episode of Channels, we talk to Jakob Zeitler - Head of R&D at Matterhorn Studios. We dive into how they use Nextflow and Seqera Platform for material science research, paving the way for cheaper and more eco-friendly products for the future ⚗️👩🏻‍🔬🔬🌎 It may not be bioinformatics, but there's a lot that's in common!

In this episode of the podcast, Geraldine and Ben talk about the Global Alliance for Genomics and Health (GA4GH) and its efforts to develop interoperability standards for pipelining infrastructure. If the words TES, WES, TRS and DRS are just as much alphabet soup to you, tune in to learn all about how organizations around the world are collaborating to streamline the process of sharing and analyzing data at scale.

In this episode of Channels, we talk to Josh Chorlton - CTO & cofounder of BugSeq Bioinformatics Inc. We talk about how BugSeq got into using Nextflow and MultiQC and the tips and tricks that they've employed to push scale and performace of their tools to the limit. We cover topics like testing and CI/CD, passing around structured data objects between Groovy and Python using Protobufs, building Docker images at scale and the ins and outs of using MultiQC for custom clinical data reports.

A recap of our current training formulas, how we got here and where we’re heading to next. Join us in this episode of the Channels podcast as Geraldine Van der Auwera and Chris Hakkaart discuss options for learning Nextflow in 2024. A recap of our current training formulas, how we got here and where we’re heading to next. Upcoming training opportunities Community Foundational Nextflow Training — Online, 5-6 March 2024 Training at Seqera Sessions — London, 21 March 2024 Training at Nextflow Summit — Boston, 21-22 May 2024 Other upcoming events of interest nf-core Hackathon — Online + Local Hubs, 18-20 March 2024