8:3. DNA Libraries and Generating cDNA

Prot —> mRNA —> cDNA —> dsDNA. (Step 2 via reverse transcriptase and step 3 via DNA Polymerase). Now the dsDNA is injected into a virus which infects bacteria to make lots of the DNA.

Denaturation (Heat to high temp), Primer Annealing (Cool slightly), Primer Extension via polymerase (Heat but not as much as step 1).

GE find us the length of a DNA Strand (how many base pairs long).

Not really sure why I did a module on this - basically you know they’re homo recessive because they have blue eyes (the recessive trait), and you know to use q BECAUSE we’re dealing with the recessive trait.

HP Equation gives P(allele frequencies). P^2 is probability of homo dominant, Q^2 is probability of homo recessive, 2pq is probability of heterozygous allele combo. P = frequency of dom allele, Q = frequency of recessive allele.

Discusses Dihybrid Crosses and the Law of Independent Assortment.

Don’t let the title fool you. This is short. Discusses complete, incomplete, and co-dominance.

Discuss genes, alleles, geno/phenotypes, and punnet squares. The Khan Academy video discusses the history (Gregor Mendel) but let’s be real, who cares.

Mutations can be good, bad, or both.

Define mutagens and then discuss endogenous and exogenous mutagens. (Endo: ROS); (Exo: intercalators, base analogues, carcinogens)