A clear picture – staining tips for gel electrophoresis

You can run the perfect agarose gel to separate your nucleic acid fragments but if you don’t stain and image the gel properly, it’s all for not. In this second installment of Mol Bio Minutes we take a look at the staining considerations for nucleic acid gel electrophoresis with Paulius Palaima, Product Manager at Thermo Fisher Scientific. He covers the range of stains and staining approaches available while calling out pros, cons and considerations for each. How these recommendations change, depending on your sample, is also covered in this approachable but informative episode. Helpful resource links mentioned in this episode:A helpful DNA stain selection guideRNA stain options and detailsEffects of dyes on gel electrophoresis properties Subscribe to get future episodes as they drop and if you like what you’re hearing we hope you’ll share a review or recommend the series to a colleague.  Visit the Invitrogen School of Molecular Biology to access helpful molecular biology resources and educational content, and please share this resource with anyone you know working in molecular biology.  For Research Use Only. Not for use in diagnostic procedures.

Topics and terms such as biosafety, biosecurity, containment, and contamination are things most of us have heard of and think about at some level, but with the pace of molecular biology moving faster than ever, these are topics with implications that are reaching farther than ever. We’re joined by Dr. Ryan Burnette and Dr. Lauren Richardson from Merrick and company for this episode, and they’re ace communicators that help walk us through the expanding horizon and implications of these topics.This conversation starts on the basic topics, like what biocontainment is and what’s needed for each of the four levels of biosafety labs, but it quickly moves beyond, shining a light on the security and containment needs for more than just the organisms. We hear about how the data and methods used to do modern molecular biology, as well as the data generated in the experiments, are equally precious and in need of protection and containment. With public health and safety on the line, and an acknowledgement that the pace of science moves faster than that of policy, we get into the idea of who really owns responsibility for protecting data. Your role might be more important than you know, so don’t miss this conversation that will make you pause and think! Subscribe to get future episodes as they drop and if you like what you’re hearing we hope you’ll share a review or recommend the series to a colleague.  Visit the Invitrogen School of Molecular Biology to access helpful molecular biology resources and educational content, and please share this resource with anyone you know working in molecular biology.  For Research Use Only. Not for use in diagnostic procedures.

Agarose gel electrophoresis is a staple method in almost all biology and biochemistry lab where separation and analysis of nucleic acids is needed. In this Mol Bio Minutes mini episode Augustė Užuotaitė, Scientist III at Thermo Fisher Scientific, covers the basics of electrophoresis with a spotlight on how different forms of DNA migrate differently in agarose gel electrophoresis.In less than 10 quick minutes, you’ll learn about the many factors that affect DNA migration rate. Augustė reviews how DNA size, sequence, and conformation all affect migration rate, and she gives some beautifully simple examples to help it all make sense. Helpful resource links mentioned in this episode:Nucleic acid gel electrophoresis – summarized in 5 easy stepsFive important considerations for the nucleic acid gel electrophoresis Subscribe to get future episodes as they drop and if you like what you’re hearing we hope you’ll share a review or recommend the series to a colleague.  Visit the Invitrogen School of Molecular Biology to access helpful molecular biology resources and educational content, and please share this resource with anyone you know working in molecular biology.  For Research Use Only. Not for use in diagnostic procedures.

Obesity is one of the most pressing health challenges of our time, with genetic and molecular factors playing a crucial role in how our bodies regulate weight. In this season opener, we explore the science behind obesity, focusing on how hormones, genetics, and brain circuits influence feeding behavior and body weight. Join us for a fascinating discussion about the interplay between molecular biology and real-world health outcomes.Our guest, Dr. Giles Yeo, is a professor of molecular neuroendocrinology at the University of Cambridge and an expert in the genetics of obesity. With decades of research experience, Dr. Yeo dives into how hormones like GLP-1 interact with the brain and how genetic mutations can affect eating behaviors. He also explains the innovative molecular biology techniques his lab uses to map brain circuits and decode the genetic influences on body weight.But this episode isn’t all about the lab. Dr. Yeo shares his journey from studying the genetics of Japanese pufferfish to becoming a leading voice in obesity research and science communication. Whether he’s decoding how Ozempic works or reflecting on the importance of good science communication, Dr. Yeo’s passion for the field—and his knack for making complex topics relatable—shines through. Subscribe to get future episodes as they drop and if you like what you’re hearing we hope you’ll share a review or recommend the series to a colleague.  Visit the Invitrogen School of Molecular Biology to access helpful molecular biology resources and educational content, and please share this resource with anyone you know working in molecular biology.  For Research Use Only. Not for use in diagnostic procedures.

The start of Season 3 is just around the corner, slated to start in January 2025. What’s the new season hold in store you might ask? Steve assures us he’ll be back hosting Season 3 and teases at topics that are coming. These include biosecurity, bioengineering, polymer degradation, genetics of obesity, CROs and how they utilize mol bio, platelet biology, and epitranscriptomics. As if that were not enough to get you excited about the new season, Steve also teases on the inclusion of Mol Bio Minutes mini episodes in the upcoming season. These short but oh, so helpful episodes will cover molecular biology lab techniques – sure to be a hit with those working in the lab. Subscribe to get future episodes as they drop and if you like what you’re hearing we hope you’ll share a review or recommend the series to a colleague.  Visit the Invitrogen School of Molecular Biology to access helpful molecular biology resources and educational content, and please share this resource with anyone you know working in molecular biology.  For Research Use Only. Not for use in diagnostic procedures.

We’re joined in this episode by Dr. Sarah Barry, Reader in Chemical Biology at King’s College London. Sarah started with an interest in biochemistry, went into organic chemistry because it was more concrete, and now applies her chemist’s approach to understanding and manipulating biosynthesis of natural products for important areas of research, including antibiotics. This insightful conversation gives a peek into the mind of a chemical biologist’s way of thinking about and approaching challenges that span biology and chemistry. We learn about the historical challenges of discovering and synthesizing natural products, but we then hear about how innovations in molecular biology are allowing researchers to revisit this field with a new approach. Sarah and her team identify and manipulate genes, express and purify proteins in the lab, and then characterize those enzymes for their abilities to drive biocatalytic transformations that are beyond challenging using traditional organic or inorganic chemistry approaches. Our conversation spans from the details of the molecular biology methods used, to the high-level applications being explored in this research, all with an ease that only someone this interdisciplinary could do. Subscribe to get future episodes as they drop and if you like what you’re hearing we hope you’ll share a review or recommend the series to a colleague.  Visit the Invitrogen School of Molecular Biology to access helpful molecular biology resources and educational content, and please share this resource with anyone you know working in molecular biology.  For Research Use Only. Not for use in diagnostic procedures.

The history of fermented foods like beer, bread, and other foods can be traced back thousands of years to ancient civilizations in China and Egypt. This ancient technology was originally used to preserve foods when refrigeration was not an option. While less common in modern industrialized civilizations, we’re now realizing that fermented foods play a major role in gut microbiome diversity, which is a biomarker for overall health. Join our conversation with Dr. John Leech, Technologist at Teagasc in County Cork, Ireland to learn all about the history and how cutting-edge technologies are being applied to research in food fermentation. John shares his story of how he found this field of research and how he’s now headlong into striving to understand and harness the power of fermented foods. We learn about the complex microbial communities that define and deliver the health benefits of these foods, but we also hear about how this biological complexity makes them inherently irreproducible. Foods like kimchi, sauerkraut, kombucha, tepache, milk kefir, and water kefir are produced from fermentation, facilitated by complex communities of microbes. Consumption of fermented foods can alter our gut microbiome, which has been shown to affect obesity, inflammation, longevity, and efficacy of drug treatments. John and his team are using qPCR, NGS, and other methods to characterize the microbial consortia used to produce these foods. They’re now working to figure out how to simplify the consortium while still delivering health benefits, all while making the process reproducible and scalable Subscribe to get future episodes as they drop and if you like what you’re hearing we hope you’ll share a review or recommend the series to a colleague.  Visit the Invitrogen School of Molecular Biology to access helpful molecular biology resources and educational content, and please share this resource with anyone you know working in molecular biology.  For Research Use Only. Not for use in diagnostic procedures.

While some of us knew a good bit about mRNA prior to 2020, we all got a crash course on mRNA technology and its prophylactic and therapeutic potential as a result of the COVID pandemic and subsequent SARS CoV-2 vaccine development. In fact, most of us have now received at least one mRNA vaccine at this point. Our guest for this episode, Dr. Christian Cobaugh, Co-founder and CEO of Vernal Biosciences, was a passionate believer in mRNA medicines well before the pandemic. Join us to hear his story and his passion for this technology. He walks us through the molecular methods by which high-purity mRNAs are now made and purified, as well as going into the lipid nanoparticle technology by which they’re commonly delivered. As a contract development and manufacturing provider, we get to learn about the state of the market and what clients of their care about today. As a seasoned expert in this space, Christian talks about the future potential of mRNA technology for applications such as personalized cancer vaccines. If you enjoy hearing smart people talk about interesting topics with a passion, you won’t want to miss this episode! Subscribe to get future episodes as they drop and if you like what you’re hearing we hope you’ll share a review or recommend the series to a colleague.  Visit the Invitrogen School of Molecular Biology to access helpful molecular biology resources and educational content, and please share this resource with anyone you know working in molecular biology.  For Research Use Only. Not for use in diagnostic procedures.

There are all sorts of molecular tests to tell if you’re infected with something specific, but what do you use when you’re not sure what you might have? You might use a thermometer as a first step, but wouldn’t it be nice if that thermometer was a bit more high tech? In this episode we meet Dr. Nick Meyerson, CEO and co-founder of Darwin Biosciences, who’s team is working to develop the “molecular thermometer of the future.” We hear about how this physicist transitioned into molecular biology and then latched onto saliva as a sample of choice to detect early molecular indicator of infection. Nick does a great job of explaining his path leading up to the founding of Darwin Bio, the challenges of founding a company in early 2020, why saliva is their sample of choice, the beautiful simplicity of isothermal amplification methods, and the lesser-known funding routes of working with government agencies like the Defense Threat Reduction Agency (DTRA). Join us as we dive into the molecular nature of non-specific detection of infections, how it’s done using no electricity, it’s potential applications, and what the future holds for this field. Nick also shares lessons learned and some insights on the value of mentors and a lifelong curiosity. Subscribe to get future episodes as they drop and if you like what you’re hearing we hope you’ll share a review or recommend the series to a colleague.  Visit the Invitrogen School of Molecular Biology to access helpful molecular biology resources and educational content, and please share this resource with anyone you know working in molecular biology.  For Research Use Only. Not for use in diagnostic procedures.

Plastics are a modern miracle of science that have helped deliver both convenience and life-saving solutions. However, we must now grapple with the challenge of immense amounts of plastics in our waste streams and environment. How do we best deconstruct plastics to reusable or more bio-friendly molecules? This is the exact challenge being addressed by the work of Dr. Elizabeth (Izzy) Bell and her team at the National Renewable Energy Lab. Our conversation with Izzy showcases her ability to summarize complex topics very concisely and understandably, which she says is a skill that is critically important in her field because it’s so interdisciplinary. Izzy summarizes the challenges they’re working to address and then walks us through the stepwise processes she and her team use to conduct directed evolution studies. These studies aim to create and characterize enzymes capable of deconstructing common plastics, first at a laboratory scale, but eventually at an industrial scale. If you’ve ever wondered about how directed evolution studies are done, and the role that molecular biology plays with them, this conversation will be sure to clarify. In addition to the great science of this episode, Izzy also helps outline what it takes to get into and be successful in her field – a great resource for anyone aspiring to get into this area of research. We hear about how interdisciplinary the field is, but how that means it’s also ripe with opportunity for those passionate about learning and making a difference. Join us for what is sure to be an informative and inspiring episode! Subscribe to get future episodes as they drop and if you like what you’re hearing we hope you’ll share a review or recommend the series to a colleague.  Visit the Invitrogen School of Molecular Biology to access helpful molecular biology resources and educational content, and please share this resource with anyone you know working in molecular biology.  For Research Use Only. Not for use in diagnostic procedures.