Episode 40: Origins of nf-core - SciLifeLab NGI

In Episode 52 of the Nextflow podcast, Phil Ewels is joined by Ziad Al Bkhetan and Steven Manos from the Australian BioCommons to explore how Nextflow and Seqera Platform are transforming bioinformatics research across Australia.Discover how the Australian BioCommons emerged as a national infrastructure investment to support molecular life sciences researchers, building digital platforms and tools that serve diverse research communities - from genome assembly to proteomics. Learn about their journey from identifying Nextflow as the leading workflow management system in Australia to successfully deploying Seqera Platform for over 90 users across 18+ research institutions.The conversation covers fascinating success stories including the collaborative development of the nf-core/proteinfold pipeline, supporting structural biology researchers with advanced protein folding tools, and the innovative Ozark community phylogenetic trees workflow. Steven and Ziad share insights on overcoming challenges like integrating web platforms with traditional HPC infrastructure at Australia's tier-one supercomputing facilities (NCI and Pawsey), and building hybrid compute environments that seamlessly span local HPC and cloud resources.Key highlights include Australia's growing contribution to the global nf-core community, culminating in their first official participation in the March 2025 nf-core hackathon with a dedicated Sydney hub. The team also discusses practical advice for other countries looking to establish national Nextflow services, emphasizing the importance of building local expertise and community engagement.For Australian researchers interested in accessing these services, visit biocommons.org.au to learn more about available platforms, training opportunities, and community resources.00:00 Podcast Ep 52: BioCommons00:06 Welcome00:41 Ziad - introduction01:36 Steven - introduction03:40 Introduction to Australian BioCommons08:13 Nextflow usage in Australia09:05 BioCommons collaboration with Seqera12:24 Compute infrastructures in Australia15:05 Nextflow support & training16:43 Highlights and lowlights19:11 Protein fold21:50 Ozard Community23:39 Automation with Seqera Platform25:52 Nextflow Communty in Australia29:27 2025 nf-core hackathon31:57 Future plans for Nextflow & Seqera Platform33:04 Tips for building a National Nextflow platform36:21 Next steps37:51 Wrap upResources:Australian BioCommons: https://biocommons.org.aunf-core office hours: https://nf-co.re/blog/2025/apac-helpdesk-2025

In Episode 51 of the Nextflow podcast, Phil Ewels and Ben Sherman discuss Nextflow's upcoming strict syntax - a significant evolution bringing clearer error messages and a more consistent language framework. They explore key changes including the separation of code from declarations, removal of for/while loops in favour of operators, and changes to type annotations. Learn how these improvements lay the groundwork for better developer experiences with features like static type checking and auto-formatting. The episode covers migration timelines, practical steps to prepare, and how these changes will ultimately lead to more robust, maintainable pipeline code. Check out the Nextflow docs for the "Updating Nextflow Syntax" guide to start preparing today.00:00 Podcast Ep51: Strict Syntax00:07 Welcome and introduction01:20 Language server background04:18 Syntax sugar is just empty calories06:50 Bespoke language syntax08:20 To DSL3 or not 2 DSL10:59 Nextflow versioning15:13 Diving into specifics16:08 Mixing statements with script declarations19:19 Better inspect command21:08 Custom Classes23:32 For loops, while and switch26:02 Standard library26:56 Type annotations28:49 addParams32:26 Process script section34:20 Moving into lib/36:36 Configuration syntax41:14 Relieving pressure from the config43:38 Timelines44:55 The gain for the pain47:54 Next steps48:43 Conclusion

In this milestone 50th episode of the Nextflow podcast, host Phil Ewels sits down with Krešimir Beštak, a PhD student and active contributor to the nf-core community, to explore an exciting frontier in bioinformatics: microscopy and spatial omics.While Nextflow is traditionally associated with genomics workflows, Krešimir shares how it’s being used to power image analysis pipelines like MCMICRO, supporting complex research into cardiovascular disease and cancer diagnostics. Based at the University Hospital Heidelberg, Krešimir discusses his transition from master’s student to PhD researcher, the translational applications of spatial proteomics, and how Nextflow enables reproducible workflows far beyond its original scope.00:00 Podcast Ep 50: Krešimir00:09 Welcome and introductions03:13 Introduction to Spatial Omics04:26 Multiplexing markers06:38 Metabolite microscopy07:23 Myocardial multiomics08:58 Microscopy data analysis11:32 nf-core/mcmicro13:05 2D vs 3D microscopy13:38 Computational bottlenecks within analysis15:43 Other nf-core imaging pipelines18:36 Downstream analysis after molkart19:53 Manual interventions23:27 Minerva25:39 Google Maps for cells27:16 Microscopy community around Nextflow30:34 How to get involved31:38 Changes in Nextflow for microscopy32:43 Nextflow Ambassador program33:17 Conclusion

Join host Phil Ewels in Episode 49 of the Nextflow Podcast as he interviews Dr. Olga Botvinnik, founder and CEO of Seanome. Olga discusses her journey from studying at MIT to founding her non-profit research institute focused on marine genomics. Discover her groundbreaking work in annotating unknown proteins and her efforts in collaborating with the Earth BioGenome Project.Olga also shares her experience with the Nextflow and nf-core communities, and her vision for developing tools to aid research in understudied ocean organisms.Join us as we chat about the vast potential of marine genomics and its applications.00:00 Podcast Episode 49: Olga Botvinnik / Seanome00:06 Welcome01:18 Olga's background07:30 Colonial tunicates09:38 Starting Seanome with Arctic clams11:07 Vision for Seanome12:46 Earth BioGenome Project13:46 Animal genomics not metagenomics14:53 Building nf-core/proteinannotator19:46 Software engineering in bioinformatics22:21 nf-core early days27:46 Bioinformatics Beyoncé30:50 Seanome future steps32:10 Final thoughts: Why the ocean34:19 Goodbyes

In this episode of the Nextflow Podcast, we dive into highlights from the recent Nextflow Summit held in Barcelona in 2024. The summit featured an incredible range of talks, demonstrations, and discussions showcasing the latest advancements in workflows, data analysis, and community collaboration. With so much exciting content to explore, we’ve picked out some of our favourite moments to share with you. From impactful new features to inspiring keynote addresses and humorous presentations, this episode offers a curated selection of clips that capture the energy and innovation from the event. Join us as we relive the summit’s best bits and discuss their significance for the Nextflow community. 00:00 Start 00:10 Podcast introduction 00:47 Introductions: Group 1 01:56 Clip 1: Wave mirroring 04:32 Clip 2: Throwing cat food 06:00 Clip 3: crisprseq 08:03 Clip 4: nf-test 10:34 Clip 5: Workflow output definition 12:39 Clip 6: Open Science Software Foundation launch 15:08 Clip 7: FAIR code with nf-core 16:58 Clip 8: Teaching with Nextflow 18:56 Clip 9: Conda lock files 21:24 Clip 10: MultiQC in scripts 22:43 Clip 11: Small Nextflow Big Nextflow 25:17 Introduction: Team 2 26:11 Clip 12: Genomics England 29:47 Clip 13: Alphafold interactive reports 33:26 Clip 14: Data Studios 35:32 Clip 15: Covid and Ebola 37:35 Clip 16: Seqera AI 41:34 Clip 17: Nextflow VSCode DAGs 44:33 Clip 18: Fusion snapshots 48:38 Clip 19: Sustainable computing 52:38 Clip 20: nf-core/tools 56:37 Clip 21: Industry best practices with nf-core 58:58 Clip 22: Federated data queries 01:01:56 Clip 23: Nextflow Ambassadors 01:05:04 Conclusion

In episode 47 of The Nextflow Podcast, Phil Ewels and Ben Sherman discuss the launch of the new Nextflow language server, a significant upgrade providing advanced code intelligence features such as code completion and error hints for VS Code users. They detail the benefits of formalizing Nextflow as its own programming language, reducing reliance on Groovy, and improving error messages and code clarity. They also cover new documentation, plans for future features like type annotations, and encourage community feedback and adoption. Find the new docs here: https://www.nextflow.io/docs/latest/vscode.html 00:00 Podcast Ep 47 - Version 2 - VSCode Extension 00:09 Welcome and introductions 00:44 Podcast rebranding 01:29 Nextflow Summit announcements 02:48 Language server background 06:27 Nextflow as a programming language 09:58 Building a language server 14:12 VS Code interactions with the language server 15:22 VS Code demo 15:50 Errors + warnings tray 17:10 Errors: Level 0 20:00 Errors: Level 1 22:32 Errors: Level 2 23:02 To def or not to def 24:46 Variable references 25:34 Number of arguments 25:57 Hover hints on workflows and processes 26:19 Code navigation 26:52 Output hints 27:17 Code peeks 27:56 Getting help 28:29 Code formatting 30:25 Nextflow schema params 32:03 Future warnings 34:02 Preview DAG 34:54 Fixing new syntax errors 35:42 Config files 37:07 Updating the plugin 37:33 Roadmap: Type checking 38:55 Conclusion

In this episode of Channels, the Nextflow podcast, host Geraldine from Seqera and her colleague Ben Sherman dive into the differences and similarities between Nextflow and the Workflow Description Language (WDL). They discuss the origins and development of WDL at the Broad Institute, the challenges of working with different workflow languages, and how Nextflow's channel-based data flow model compares to WDL's approach. The conversation covers key features, type systems, and runtime specifications, highlighting the strengths and limitations of both languages. Ben hints at upcoming improvements and enhancements for Nextflow, aimed at making the language more robust and user-friendly. The episode offers valuable insights for bioinformaticians and developers navigating the complexities of workflow management.

In this episode of Channels, the Nextflow podcast, join us as we explore Nextflow and AI. Our special guest, Sasha from Seqera, shares his remarkable journey from the consumer tech industry to bioinformatics. Learn about the birth of TinyBio, its innovations in bioinformatics AI applications, and its recent acquisition by Seqera. We delve into AI concepts, the challenges faced, and the future of integrating AI with Nextflow. Tune in to discover how advancements in AI are reshaping the bioinformatics landscape. 00:00 Podcast 45: Tinybio : Nextflow x AI 00:09 Introduction 00:34 Sasha's background 03:17 How tinybio started 05:57 Tinybio co-founder: Vishal Patel 06:35 tinybio chat public launch 09:20 Failed feature launches 10:39 Running the code too 12:46 User trust 14:11 Accessing files via chat 15:08 Experiment idea generator 17:16 Pipeline generator 20:25 AI concepts: Foundational models 22:36 AI concepts: RAG 24:53 AI concepts: Langchain 26:37 AI concepts: Fine tuning 29:14 Challenges in bioinformatics AI 31:53 AI concepts: Q&A training 33:09 Motivation for joining Seqera 34:43 What happens to tinybio now? 35:36 Future plans 38:20 AI and open-source 40:50 Find more updates 41:37 Wrap up

In episode 44 of the Nextflow Channels podcast, Phil Ewels welcomes Alex Pelzer— a key figure in the Nextflow and nf-core community. Alex shares his journey from academia to the pharmaceutical industry, highlighting the critical role of regulatory frameworks in bioinformatics. 🌟 Key Highlights: The transition from working at QBiC to Boehringer Ingelheim. The importance of maintaining high-quality, reproducible pipelines in bioinformatics. Understanding regulatory requirements and their impact on clinical trials. How regulatory standards and open-source initiatives like @nf-core are shaping the future of bioinformatics. Alex also discusses the formation of the @nf-core Regulatory Special Interest Group, which aims to bring together experts in the field to establish and promote best practices for ensuring pipeline quality and compliance with regulatory standards. Looking forward to seeing many of you at the upcoming #NextflowSummit in Barcelona this October! We’ll be working with this #regulatory group during the nf-core hackathon. 00:00 Episode 44: Regulatory revolution 00:06 Introductions 00:33 Upcoming Nextflow Summit Announcement 01:34 Alex’s Background and Early Days with Nextflow 04:41 Formation and Growth of nf-core 06:18 Transition to Pharma and Regulatory Work 08:56 Regulatory Challenges and Practices in Bioinformatics 13:30 Tool Selection and Quality Assurance 15:23 Evaluating Software Trustworthiness 17:08 Overhead in Academia vs. Industry 18:11 Downstream analysis 20:57 Regulatory Special Interest Group 25:28 Special Interest Groups in nf-core 28:59 Future Directions and Community Involvement 30:15 Open source contributions in pharma 33:27 Conclusion and Upcoming Events

In this episode we explore the new features of Seqera's Data Studios and Data Explorer, with Phil Ewels, Rob Newman and Rob Syme from Seqera. Discover how to use these tools for troubleshooting Nextflow pipelines, tertiary analysis and Nextflow development. We discuss the pain points that led to the creation of Data Studios and how it's designed to allow scientists to interactively and collaboratively work with data and complex workflows, without having to move large datasets around. Rob Syme wows us with another fantastic practical demonstration, setting up and using Data Studios to write and test a Nextflow pipeline in VSCode running on the cloud in a Data Studio environment, including running the Nextflow CLI with task submission to AWS Batch. We cover features like session persistence to save work states, and upcoming custom container support for your own specialized applications. Learn how these tools can enhance your computational biology projects and make seamless cloud integration a reality. 00:00 Channels Podcast 43: Data Studios 00:26 Introductions 01:54 Data Studios 04:51 Move the compute to the data 06:13 Real-time collaboration 06:47 Data Explorer 09:41 Access to public data 10:45 Data Explorer demo 13:56 Data Studios setup 20:17 Session persistance 22:52 Data Studios RStudio demo 28:24 Nextflow development in Data Studios 36:17 Future development 37:01 Custom containers 40:01 Boston Summit demo 44:01 Lifetime management 47:14 Wrap up