Episode 6: AAVs - From Basics to Breakthroughs

What exactly is gel permeation chromatography (GPC), and why is it one of the most powerful tools for polymer characterization? In this episode of the Chromatography Experts Podcast, we explore the fundamentals and evolution of GPC with a practical discussion on how scientists use the technique to determine molecular weight distributions, evaluate polymer properties, and gain insight into material performance. Whether you're new to GPC or an experienced chromatographer, this conversation offers valuable insights into the science, best practices, and future direction of one of the most widely used size-based separation techniques. 🎙️ Subscribe to Chromatography Experts for conversations with scientists and industry specialists covering separation science, analytical technologies, bioprocessing, and the latest advancements shaping the field.

In this episode of Chromatography Experts, Patrick Endres hosts Elena Kumm and Balu Guduri, as they discuss the challenges of moving from process development to GMP manufacturing and the growing demand for large-scale pre-packed chromatography columns. Topics include: The challenges of scaling from development to manufacturing When column packing becomes a business and regulatory risk The performance data behind the SkillPak™ 1k MAX launch Chromatographic consistency, robustness, and reproducibility at 1 L scale The value of pre-packed columns compared to in-house or third-party packing Early customer feedback and what success looks like for manufacturing-scale chromatography Whether you're involved in process development, scale-up, or downstream purification, this conversation provides valuable insights into reducing risk and improving confidence in manufacturing-ready chromatography solutions.

In this episode of Chromatography Experts, Patrick Endres of Tosoh Bioscience is joined by Steffi Stephen and Daniele Addis from Axolabs for an in-depth discussion on oligonucleotides. Together, they explore what oligonucleotides are, how they are produced, and the key steps involved in moving from synthesis to a purified therapeutic product. The conversation covers emerging applications such as antibody-oligonucleotide conjugates (AOCs), as well as the purification and analytical challenges associated with developing oligonucleotide-based therapies. Steffi and Daniele share insights into the critical role chromatography plays in addressing these challenges. Whether you're new to oligonucleotides or looking to expand your knowledge of this rapidly growing field, this episode offers valuable perspectives on the technologies, challenges, and partnerships driving the future of nucleic acid therapeutics.

In this episode of Chromatography Experts, we take a deep dive into the science and advantages of pre-packed chromatography columns. Our experts discuss how column packing impacts performance, reproducibility, and process efficiency, and why pre-packed solutions continue to gain traction in bioprocessing and pharmaceutical manufacturing. Topics covered include: The GMP advantages of pre-packed chromatography columns • What defines a "well-packed" column and why packing quality is critical • The science behind column packing and chromatographic performance • How pre-packed columns help improve consistency, reduce risk, and streamline operations • Key considerations when selecting a chromatography column for your process Subscribe to Chromatography Experts for more conversations on the science, technologies, and innovations shaping modern chromatography.

Getting started with MALS doesn’t have to be complex. In this episode of the Chromatography Experts podcast, we break down the fundamentals of Multi-Angle Light Scattering (MALS), from instrumentation and setup to real-world troubleshooting and data interpretation. You’ll learn: What MALS is and how it integrates with chromatography systems The detectors and setup required for accurate measurements How sample quality impacts your results (and common pitfalls to avoid) Key troubleshooting strategies when results don’t look right Best practices for maintaining performance and interpreting data with confidence Whether you're new to MALS or looking to refine your workflow, this episode is packed with practical insights to help you get consistent, high-quality results.   Interested in learning more about MALS? Find more here: https://www.tosohbioscience.com/US-EN-separations/lens-mals-education?utm_source=social-organic&utm_

We are back with another episode of Chromatography Experts! This time, we focus on Multi-Angle Light Scattering (MALS) with Corey Meadows and Landry Bennett. In this episode, we break down the fundamentals of MALS, explore real-world applications, and explain why light scattering remains such a powerful analytical technique.

Prepacked chromatography columns are changing how scientists approach purification—making scale‑up faster, more consistent, and more reliable than ever before. In this episode, we explore prepacked chromatography columns… what they are, how they’re manufactured, and why they’ve become a go‑to solution from early process development through GMP production. Our experts Kevin O’Donnell and Phu Duong discuss performance consistency and how pre-packed formats help reduce variability and save time in the lab and manufacturing. Topics include: Prepacked vs. batch columns Scale‑up and reproducibility Time, cost, and operational efficiencies Best practices for implementation Perfect for scientists, engineers, and bioprocessing professionals looking to streamline purification without compromising performance. Learn more today: https://bit.ly/4bUcROm

Join host Abbie Breton and special guest Yuliya McAnany on Chromatography Experts as we explore AAV purification, cell & gene therapy advancements, and a new method from Tosoh that’s reshaping how researchers separate full and empty AAV capsids. We break down the science behind our recent paper, ‘Preparative choline-based anion-exchange chromatography for enrichment of full adeno-associated virus capsids’ and reveal the results, up to 90% full capsids with 75% recovery. You’ll also hear insights on scalability, method adaptation for other viral vectors, and expert advice for improving purification workflows. If you work with AAVs, this is an episode you don’t want to miss. The paper can be found here: https://www.sciencedirect.com/science/article/pii/S0021967325006636

Episode 6 of Chromatography Experts is out now! 🎙️ AAVs - From Basics to Breakthroughs What are AAVs, and why are they revolutionizing gene therapy? In this episode, we break down the science, highlight a breakthrough analytical method from Tosoh, and explore the future of AAVs in clinical development. 🔬 Whether you're in the lab or just curious about biotech innovation, this episode is packed with insights.

🔬 Episode #5 – Exploring Chromatography Resin Selection In this episode, we dive into selecting the right chromatography resins for bioprocessing applications and how important it is to have a trusted vendor in today’s market. 🎙️ Join our #ChromatographyExperts Phu Duong and Anson Lewis as they discuss Key factors influencing resin selection Performance comparisons across resin types Real-world case studies and lessons learned Upcoming innovations in resin technology 📌 Perfect for scientists, process engineers, and anyone curious about the science behind purification strategies. #Chromatography #Bioprocessing #ResinSelection #AAV #mAb #DownstreamProcessing #Podcast