325: Microbes Making Methane

TWiM explains how to recode E. coli so it uses only one stop codon, and an exploration of the mechanisms of bacterial adhesion within dental plaque. Hosts: Vincent Racaniello, Michael Schmidt, Petra Levin and Michele Swanson. Become a patron of TWiM. Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Links for this episode Engineering E. coli with one stop codon (Nature) Bacterial adhesion in dental plaque (mBio) Microbial complexes in subgingival plaque (J Clin Periodontol) Bacterial clusters in periodontal and peri-implant diseases (Microorg) Take the TWiM Listener survey! Send your microbiology questions and comments (email or recorded audio) to [email protected]

TWiM explains Pasteur’s relentless hunt for microbes in the air, and how bacteria hunt for prey by ixotrophy - using grappling hooks! Hosts: Vincent Racaniello, Michael Schmidt, Petra Levin and Michele Swanson. Become a patron of TWiM. Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Links for this episode Pasteur’s hunt for microbes in the air (NYTimes) Bacterial predation by ixotrophy (Science) Enter the pirates (STC) Take the TWiM Listener survey! Send your microbiology questions and comments (email or recorded audio) to [email protected]

TWiM describes Shigella infection is facilitated by interaction of human enteric α-defensin 5 with a colonic epithelial receptor, and an amino acid change in RNA polymerase that leads to resistance to β-lactams by preventing dysregulation of amino acid and nucleotide metabolism Hosts: Vincent Racaniello, Michael Schmidt, Petra Levin and Michele Swanson. Subscribe to TWiM (free) on Apple Podcasts, Spotify, Android, RSS, or by email. Become a patron of TWiM. Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Links for this episode National Black HIV/AIDS awareness day (CDC) Shigella infection and human enteric alpha-defensin 5 (Nature Microbio) RNA polymerase amino acid change and resistance to beta-lactams (Cell Rep) Take the TWiM Listener survey! Send your microbiology questions and comments (email or recorded audio) to [email protected]

TWiM explains the remarkable abilities of bacterial ice nucleating proteins to promote freezing of water, and cryoprotective proteins produced by worm microbiomes that prevent freezing. Hosts: Vincent Racaniello, Michael Schmidt, Petra Levin and Michele Swanson. Become a patron of TWiM. Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Links for this episode Worm microbiomes produce crytoprotective proteins (Sci Adv) Biological antifreeze in Antarctic fishes (Sciece) Enhancement of bacterial ice nucleators (PNAS) Take the TWiM Listener survey! Send your microbiology questions and comments (email or recorded audio) to [email protected]

TWiM explores the discovery of microbial enzymes, PETases, that can degrade ubiquitous plastics, and how exogenous peptidoglycan is a danger signal to trigger biofilm formation. Hosts: Vincent Racaniello, Michael Schmidt, Michele Swanson. Guest Mark O. Martin. Subscribe to TWiM (free) on Apple Podcasts, Spotify, Android, RSS, or by email. Become a patron of TWiM. Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Links for this episode Discovery of PET depolymerases (Science) Exogenous peptidoglycan triggers biofilm formation (Nat Micro) What is a biofilm? (Vimeo) Peptidoglycan as a PAMP/MAMP (Immunol Rev) Light organ formation triggered by peptidoglycan (Science) Take the TWiM Listener survey! Send your microbiology questions and comments (email or recorded audio) to [email protected]

TWiM reveals that record high atmospheric methane growth has been driven by microbes, and the cecum as an adaptive niche for Salmonella typhi. Hosts: Michael Schmidt, Petra Levin and Michele Swanson. Subscribe to TWiM (free) on Apple Podcasts, Spotify, Android, RSS, or by email. Become a patron of TWiM. Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Links for this episode Why a potent greenhouse gas is rising (WaPo) Microbial emissions drove record high atmospheric methane growth (PNAS) Salmonella biofilm formation in the cecum (mBio) Typhoid Mary (The Collector) Take the TWiM Listener survey! Send your microbiology questions and comments (email or recorded audio) to [email protected]

From ASMCUE 2024, the conference on undergraduate education, TWiM speaks with Becky, Melanie, and Katriana about their careers and how they use TWiM in undergraduate microbiology education. Hosts: Michael Schmidt and Mark O. Martin Guests: Becky Seipel-Thiemann, Melanie Melendrez-Vallard, and Katriana Popichak Subscribe to TWiM (free) on Apple Podcasts, Spotify, Android, RSS, or by email. Become a patron of TWiM. Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Links for this episode Take the TWiM Listener survey! Send your microbiology questions and comments (email or recorded audio) to [email protected]

TWiM describes how to make concrete more ‘green’ by using microbes, and bacterial bioluminescence as an important regulator of multitrophic interactions in the soil. Hosts: Vincent Racaniello, Michael Schmidt, Michele Swanson and and Mark O. Martin. Subscribe to TWiM (free) on Apple Podcasts, Spotify, RSS, or by email. Become a patron of TWiM. Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Links for this episode Construction nanobiotechnology for concrete (Appl Env Micro) Self-healing concrete (Sci Tot Environ) Microbe-inspired self-healing concrete (Front Struct Civil Eng) Bacterial bioluminescence regulates multitrophic interactions in the soil (Cell Rep) Take the TWiM Listener survey! Send your microbiology questions and comments (email or recorded audio) to [email protected]

TWiM explains how ticagrelor alters the membrane of S. aureus and enhances the activity of vancomycin and daptomycin without eliciting cross-resistance, and the development of a novel continuous disinfectant technology that decreases healthcare-associated infections in ICUs by 70%. Hosts: Vincent Racaniello, Michael Schmidt, and Michele Swanson. Become a patron of TWiM. Links for this episode Ticagrelor and S. aureus (mBio) Novel disinfectant technology (Am J Inf Control) UVC-LED to inactivate foodborne pathogens (Appl Envir Micro) UV disinfection systems (ACS Photonics) High-touch surfaces in specialized patient care area (CDC) Take the TWiM Listener survey! Send your microbiology questions and comments (email or recorded audio) to [email protected]  

TWiM focuses on recent foodborne outbreaks of bacterial infections, and how nanopore sequencing technology can be used to identify pathogenic microbes and antimicrobial resistance genes in food products. Hosts: Vincent Racaniello, Michael Schmidt, Petra Levin and Michele Swanson. Become a patron of TWiM. Links for this episode Foodborne outbreaks (CDC) Race to nourish a warming world (Gates Foundation) Nanopore sequencing of foods (Food Microbiol) How is Oxford Nanopore used? (YouTube) Introduction to Nanopore sequencing (YouTube) Methods for detecting foodborne pathogens (Appl Micro Biotech) Take the TWiM Listener survey! Send your microbiology questions and comments (email or recorded audio) to [email protected]